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KMID : 0380020120270050313
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 5 p.313 ~ p.318
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An Increased Intracellular Calcium Ion Concentration in Response to Dimethyl Sulfoxide Correlates with Enhanced Expression of Recombinant Human Cyclooxygenase 1 in Stably Transfected Drosophila melanogaster S2 Cells
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Chang Kyung-Hwa
Park Jong-Hwa
Kim Do-Hyung
Chung Ha-Young
Hwangbo Jeon
Lee Hyun-Ho
Lee Hee-Young
Shon Dong-Hwa
Kim Won-Yong
Chung In-Sik
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Abstract
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Dimethyl sulfoxide (DMSO) increased the intracellular calcium ion concentration in stably transfected Drosophila melanogaster S2 cells expressing recombinant cyclooxygenase 1 (COX-1). DMSO did not increase the Drosophila NOS (dNOS) transcript level in calcium chelator-treated cells. Expression of recombinant COX-1 due to DMSO was diminished in cells treated with calcium chelators or channel blockers. Our results indicate that an increased intracellular calcium ion concentration due to DMSO is associated with up-regulation of the dNOS gene, leading to enhanced expression of COX-1.
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KEYWORD
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recombinant cyclooxygenase 1, dimethyl sulfoxide, Drosophila melanogaster S2 cells, calcium ion
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